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M94A3123.TXT
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1994-10-25
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Document 3123
DOCN M94A3123
TI Evidence of an in vitro association of HIV with other DNA viruses.
DT 9412
AU Cisterna R; Campelo C; Gorrino MT; Malave C; Sarria L;
Fernandez-Aranguiz A; Lardelli P; Sunen E; Madariaga L; Immunology,
Microbiology and Parasitology Dept. University of the; Basque Country,
Spain.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):149 (abstract no. PB0023). Unique
Identifier : AIDSLINE ICA10/94369452
AB Several authors have focused their attention on the possibility that
cofactors may be important in the pathogenesis of acquired
immunodeficiency syndrome (AIDS). To investigate the association of
Human Immunodeficiency Virus (HIV) with various DNA viruses e.g.
Hepatitis B Virus (HBV), Citomegalovirus (CMV) and Epstein-Barr Virus
(EBV), simultaneous determinations of HIV p24 antigen (HIVp24Ag), HBV
surface antigen (HBsAg) and DNA, CMV DNA and EBV DNA were performed in
lymphocyte culture supernatants from 54 individuals at risk of HIV
infection. In 25 patients all viral determinations cited above could be
done: in 10 patients, simultaneous expression of all viruses was
detected, 12 patients resulted negative for expression of all viruses
and 3 resulted positive for some other virus in absence of HIVp24Ag. In
29 patients determination of all viruses was not possible: 14 were
positive for HIVp24Ag expression and in all of them simultaneous
expression of some other virus was detected, 15 were HIVp24Ag negative
in culture supernatants but in 13 of them some other viral expression
was always detected being only the remaining 2 patients negative for all
other virus tested. Thus, HIV expression in lymphocyte culture
supernatants never occurred alone as expression of some other virus was
always detected in the 24 patients expressing HIV antigen in vitro from
the 54 patients tested. Furthermore, 16 patients were positive for the
determination of some other viral expression in the absence of HIV
expression and 14 were negative for all viruses tested.
DE Cells, Cultured DNA Viruses/*ISOLATION & PURIF DNA, Viral/*ANALYSIS
Hepatitis B Surface Antigens/ANALYSIS Human HIV/*ISOLATION & PURIF
HIV Core Protein p24/ANALYSIS HIV Infections/EPIDEMIOLOGY
Lymphocytes/*MICROBIOLOGY Risk Factors MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).